英语翻译Experimental SectionSynthesis of Mesoporous Silica Nanop

英语翻译
Experimental Section
Synthesis of Mesoporous Silica Nanoparticle (MSN).Dye-
doped MSN was synthesized by modifying a previously reported
method.
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Rhodamine B isothiocyanate (RITC,Aldrich) (5 mg) or
fluorescein isothiocyanate (FITC,Aldrich) (4 mg) was reacted with
44µL of 3-aminopropyltriethoxysilane (APS,Sigma,98%) (molar
ratio of dye:APS ) 1:10) in 1 mL of ethanol overnight in the dark.
NaOH (0.35 mL,2 M) was added to 50 mL of cetyltrimethylam-
monium bromide (CTAB,Acros,99+%) solution (0.1 g in 50 mL
of water).The mixture was heated to 70 °C,followed by the
addition of 0.5 mL of tetraethylorthosilicate (TEOS,Acros,98%)
and 50 µL of APS-modified dye solution.After 1 min,0.5 mL of
ethyl acetate (EtOAc,Samchun,99.5%) was added,and the
resulting mixture was stirred at 70 °C for 30 s and then aged for
2 h.The precipitate was collected by centrifugation and washed
with water and ethanol four times.Finally,the pore-generating
template,CTAB,was removed by refluxing in acidic ethanol
solution.A JEM-2010 transmission electron microscope (JEOL)
was used for the transmission electron microscopy (TEM) analysis.
The average size of MSN was estimated by measuring the sizes of
over 200 particles.
Surface Modification of MSN.The surface of MSN was
functionalized with amine groups by treatment with APS.MSN
(0.1 mg) was dispersed in 10 mL of ethanol.The solution was
refluxed for 3 h,followed by the addition of 40 µL of APS.After
centrifugation and washing with ethanol,amine-functionalized MSN
was redispersed in 5 mL of ethanol.
Ligand Exchange of Fe 3O4
Nanocrystals.Monodisperse Fe3O4
nanocrystals capped with oleic acid were synthesized in organic
solution via previously reported procedures.
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2-Bromo-2-methyl-
propionic acid (BMPA,Aldrich,98%) (0.5 g) and citric acid
(Samchun) (0.05 g) were dissolved in a mixture of chloroform and
DMF (50/50 v,15 mL).
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The synthesized nanocrystals (15 mg)
were dispersed in the BMPA solution and stirred overnight at
30°C.Finally,the ligand-exchanged nanocrystals were retrieved
by centrifugation and redispersed in 5 mL of ethanol.
Synthesis of Fe
3O4
-MSN and Surface Modification with PEG.
The amine-functionalized MSN dispersion (1 mL) was reacted with
5 mL of the ligand-exchanged Fe
3O4
nanocrystals dispersion.The
Fe
3O4
-MSN was retrieved by centrifugation and washed with
ethanol to remove excess Fe
3O4
nanocrystals.The resulting Fe
3O4
-
MSN was mixed with 25 mg of methoxy poly(ethylene glycol)
succinimidyl glutarate (mPEG-SG,MW 5000,Sunbio) dissolved
in 5 mL of ethanol.The mixture was stirred overnight at 30 °Cto
induce covalent bonding between residual amine groups on the
surface of MSN and succinimidyl groups of the PEG.After removal
of unreacted mPEG-SG by centrifugation,25 mg of methoxy

试验段合成的介孔二氧化硅纳米粒子（质谱）.染料—合成了掺杂软件修改先前报告方法.8异硫氰酸罗丹明B（瑞达国贸,奥德里奇）（5毫克）或异硫氰酸荧光素（荧光,奥德里奇）（4毫克）反应44µ升3（黄芪多糖,西格玛,98%）（摩尔比染料：黄芪多糖）1 :10）在1毫升乙醇在黑暗.氢氧化钠（0.35毫升,2米）加入50毫升的cetyltrimethylam—溴化铵（铵,苯,99 + %）溶液（0.1克50毫升水）.混合物被加热到70摄氏°,其次是加入0.5毫升的正硅酸乙酯（正硅酸乙酯,苯,98%）50µ升aps-modified染料溶液.1分钟后,0.5毫升乙酸乙酯（乙酸乙酯,samchun,99.5%）被添加,和由此产生的混合物搅拌70°为30,年龄2小时沉淀,离心收集和清洗与水和乙醇的四倍.最后,该pore-generating模板,表面活性剂,去除酸性乙醇回流解决方案.一个教学透射电子显微镜（日本电子公司）用于透射电子显微镜（透射电镜）分析.平均大小的软件估计测量尺寸200粒子.表面改性的软件.表面干的是功能与胺组的治疗黄芪多糖.微软的（0.1毫克）是分散在10毫升乙醇.解决办法是回流3小时,其次是增加了40µ升的AP.后离心,洗涤用乙醇胺,微软本发明涉及了5毫升乙醇.配体交换铁3o4纳米晶体.分散的Fe3O 4纳米晶包覆油酸合成有机物通过先前报告的程序.132-bromo-2-methyl—丙酸（bmpa,奥德里奇,98%）（0.5克）和柠檬酸（samchun）（0.05克）溶解在混合的氯仿和二甲基甲酰胺（50/50伏,15毫升）.14合成的纳米晶（15毫克）分散在bmpa溶液搅拌过夜30°C .最后,ligand-exchanged纳米晶体的检索离心分离和再分散在5毫升乙醇.合成铁3o4-与表面改性聚乙二醇.微软的胺官能分散（1毫升）反应5毫升的ligand-exchanged铁3o4纳米晶体色散.本铁3o4-微软被检索由离心洗乙醇消除多余的铁3o4纳米晶体.由此产生的铁3o4—微软是混有25毫克甲氧基聚（乙烯乙二醇）琥珀戊二酸（mpeg-sg,分子量5000,sunbio）溶解在5毫升乙醇.将混合物搅拌一夜之间在30°首席技术官诱导共价键之间的残余胺基团的表面和琥珀的群体的聚乙二醇.搬迁后未mpeg-sg离心,25毫克甲氧基分享到