0.1%的过氧乙酸的杀菌效果,持续120秒,能杀灭多少细菌?若此浓度灭菌效果要达到99%以上需要多少时间

0.1%的过氧乙酸的杀菌效果,持续120秒,能杀灭多少细菌?若此浓度灭菌效果要达到99%以上需要多少时间

要看在什么环境下,如果要杀菌的地方有很强的还原性,效果就差,例如有大量蛋白质或多糖存在,会消耗过氧乙酸,这时就需要提高浓度,单纯延长时间并不能起作用.对手的消毒用0.2%的溶液擦拭1－2分钟,再用清水洗净；如对物体表面进行消毒,可用浓度为0.2%－1%的过氧乙酸稀溶液,擦拭后保持30分钟,即能达到杀菌目的.如果表面有大量蛋白粘液则需要提高浓度或增大用量.
再问： 杀菌环境无强还原性物质，本身含有的微生物数量也不是很多，
再答： The study by Shimura, et al. was a case control study, developed on a flowchart. Six sterile glasses surfaces (40x30cm) were contaminated with a suspension with 104 cfu/ml MRSA by spreading with a sterile spatula and left to dry for 10 minutes. The efficacy of disinfection products were measured by imprinting rodac plates with Trypticase Soy Agar holding for 1 minute against the surface, before and after the disinfection procedure. The glass surface was divided in three parts and each part was cleaned three times. A sterile microfiber cloth (40x38cm) made from 10 percent polyester, 20 percent polypropylene and 70 percent viscose was folded three times, with a total of 16 sides, but using nine sides only; they were moistened with 50ml of 0.1 percent peracetic acid or 70 percent ethyl alcohol to clean three surfaces each. Sterile gloves were used and imprints from both hands were made on rodac plates, holding for 15 seconds. Plates were incubated at 37°C for 48 hours. The median of surface contamination before and after disinfection with peracetic acid was 3.55 cfu/ml and 0 cfu/ml, for ethyl alcohol, 4.26 cfu/ml and 0 cfu/ml respectively. Imprints of gloves after both disinfections had no growth (0 cfu/ml). The test showed a bacterial load reduction although a non-significant (p>0.05) result comparing solutions.