英语翻译When the tails combine at the same interface by means of

英语翻译
When the tails combine at the same interface by means of back-folding(Figure 1A),a lamellar bilayer vesicular morphology is attained(DNA condensing morphology),whereas partial unfolding leads to cone-shaped molecules which associate in the H11 phase(Figure 1B).
The completely unfolded conformation of the Sunfish materials by Holopainen and Kinnunen as intermediate in fusion and hemifusion processes.In this conformation,the Sunfish antennae could potentially facilitate molecular interaction processes at the cell surface and the transport of DNA across the cell membrane.The ability of Sunfish amphiphiles to attain a nonbilayer packing is a function of the interplay between the two alkyl chains,determined by their length and presence of unsaturation(s).Therefore,modifications in length and unsaturation of the alkyl chains have been introduced and their influence on the transfection activity and toxicity has been evaluated(1a-1f,Table 1).
The current study is focused on a detailed identification of the molecular features of the Sunfish amphiphiles and the corresponding complexes with DNA,in particular with regard to the preference for nonbilayer packing.The Sunfish amphiphiles were selected in such a way that the effect of small structural variations on the aggregation behavior could be identified by comparison.
Langmuir-Blodgett film balance,P-and H-NMR,cryoTEM,SAXS,and light scattering studies have been performed applying conditions optimal for in vitro transfections.The morphological data obtained for the various lipid assemblies and DNA-lipid complexes were then correlated with the in vitro transfection capacity,using COS-7 cells.
The Sunfish amphiphiles were prepared from 4-picoline and the appropriate alkyl iodides in a three-step procedure according to the methods published previously.For the current study,an array of amphiphiles(1a-1f.Table1)was chosen,differing in both tail length and unsaturation of the tails.DOPE was purchased from Avanti Polar Lipids,Inc(Alabaster,AL).Nile Red was obtained from ACROS(Landameer,The Netherlands).Asample of 7.1-kilobase DNA was isolated feom Escherichia coli using the Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands).The plasmid concentration was determined spectrophotometrically by measuring the absorption at =260nm using the relation 1.0A=50ug/mL.Typically,the A260/A280 values were - 1.85.All other chemicals were of the highest available grades.

这应该是生物化学方面的文献,涉及不少胶体化学的东西
最好还是自己练练,怎么说也是锻炼
When the tails combine at the same interface by means of back-folding(Figure 1A),a lamellar bilayer vesicular morphology is attained(DNA condensing morphology),whereas partial unfolding leads to cone-shaped molecules which associate in the H11 phase(Figure 1B).
当尾端通过折叠的方法（图1A）并到同一个界面时,将得到双层的囊泡形貌（DNA凝聚的形貌）.然而,部分展开得到的则是锥形分子,呈H11相.
The completely unfolded conformation of the Sunfish materials by Holopainen and Kinnunen as intermediate in fusion and hemifusion processes. In this conformation,the Sunfish antennae could potentially facilitate molecular interaction processes at the cell surface and the transport of DNA across the cell membrane.
通过在融合与半融合过程中使用霍洛派宁和金努宁作中间体的办法,可得到Sunfish材料完全展开的构象（这句比较奇怪）.这种构象下,Sunfish触角能够使得在细胞表面,以及跨越细胞膜的DNA传递过程中的分子相互作用易于进行.


The ability of Sunfish amphiphiles to attain a nonbilayer packing is a function of the interplay between the two alkyl chains,determined by their length and presence of unsaturation(s).Therefore,modifications in length and unsaturation of the alkyl chains have been introduced and their influence on the transfection activity and toxicity has been evaluated(1a-1f,Table 1).
Sunfish两亲化合物可以形成非双层结构的堆积,这与两个烷基链间的相互作用有关（这种相互作用由烷基链的长度与不饱和度决定）.因此,我们在此采用烷基链长度与不饱和度不同的Sunfish两亲化合物,并对其转染活性与毒性进行比较.
The current study is focused on a detailed identification of the molecular features of the Sunfish amphiphiles and the corresponding complexes with DNA,in particular with regard to the preference for nonbilayer packing. The Sunfish amphiphiles were selected in such a way that the effect of small structural variations on the aggregation behavior could be identified by comparison.
当前研究致力于对Sunfish两亲化合物与相应DNA复合物的分子特征,尤其是对非双层堆积结构的具体分析.我们依据可通过比较微小的结构变化对其聚集行为的影响的原则,来选择Sunfish两亲化合物,进行研究.
Langmuir-Blodgett film balance,P-and H-NMR,cryoTEM,SAXS,and light scattering studies have been performed applying conditions optimal for in vitro transfections. The morphological data obtained for the various lipid assemblies and DNA-lipid complexes were then correlated with the in vitro transfection capacity,using COS-7 cells.
在适于体外转染的条件下,进行了L-B膜平衡、核磁共振磷谱与氢谱、冷冻透射电子显微镜、小角X射线散射以及光散射测试.通过使用COS-7细胞,我们将各种脂类聚集体与DNA-脂类复合物的形态学数据与体外转染能力相关联.
The Sunfish amphiphiles were prepared from 4-picoline and the appropriate alkyl iodides in a three-step procedure according to the methods published previously. For the current study,an array of amphiphiles(1a-1f.Table1)was chosen,differing in both tail length and unsaturation of the tails. DOPE was purchased from Avanti Polar Lipids,Inc(Alabaster,AL).Nile Red was obtained from ACROS(Landameer,The Netherlands).Asample of 7.1-kilobase DNA was isolated feom Escherichia coli using the Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands).The plasmid concentration was determined spectrophotometrically by measuring the absorption at =260nm using the relation 1.0A=50ug/mL.Typically,the A260/A280 values were - 1.85.All other chemicals were of the highest available grades.
根据前人的报道,Sunfish （太阳鱼型）两亲化合物的制备是以4-甲基吡啶与适当的烷基碘为起始原料,通过三步法得到.我们的研究所选定的一系列两亲分子（1a-1f.表1),其尾端的长度与不饱和度均不相同.DOPE由Avanti Polar Lipids,Inc(Alabaster,AL)购得,尼罗红则是从ACROS(Landameer,The Netherlands)获取.具有7100个碱基对的DNA样品是从埃希氏菌属线团分离出来的,来自Sigma-Aldrich GenElute HP Maxiprep kit(Sigma-Aldrich,Zwijndrecht,The Netherlands). 质粒浓度由光谱数据测定,具体方法是测量260 nm处的吸收峰强度（按1.0A=50ug/mL换算得到）.一般地,260nm的吸收强度 / 280 nm处的吸收峰强度为1.85.其它试剂的纯度都是市面上可获得的最高纯度.